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Microbiology and Biotechnology Letters

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Microbial Biotechnology (MB)  |  Synthetic Biology and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2021; 49(3): 298-304

https://doi.org/10.48022/mbl.2102.02004

Received: February 4, 2021; Revised: April 14, 2021; Accepted: April 15, 2021

무-유도인자 단백질 발현 시스템을 이용한 재조합 시아노박테리아의 광합성 스쿠알렌 생산 평가

Evaluation of Photosynthetic Squalene Production of Engineered Cyanobacteria Using the Chemical Inducer-Free Expression System

Sun Young Choi1, 2, 3* and Han Min Woo1, 2*

1Department of Food Science and Biotechnology, 2BioFoundry Research Center, Sungkyunkwan University (SKKU), Suwon 16419, Republic of Korea 3SOL INC. , BK Tower 2F, Seoul 05836, Republic of Korea

Correspondence to :
Sun Young Choi,     sychoi@sol.re.kr
Han Min Woo,         hmwoo@skku.edu

Photosynthetic conversion through cyanobacteria and microalgae is an increasingly serious concern in the global warming crisis. Many value-added substances are produced through strain improvement, and much research and development is being conducted to determine its potential as an actual industrial strain. Economic barriers throughout processing production can be overcome to produce value-added chemicals by microalgal strains. In this study, we engineered cyanobacteria strains for the photosynthetic production of squalene and confirmed the continuous cultivation of CO2 and light conditions. The free-inducer system of gene expression was developed at the cyanobacterial strains. Then, the squalene production level and growth of the recombinant cyanobacteria were analyzed and discussed. For bio solar-cell factories, the ability to regulate genes based on the free-inducer gene expression system promotes metabolic engineering research and construction to produce value-added chemicals.

Keywords: Cyanobacteria, squalene, IPTG free-inducer, Synechococcus elongatus PCC 7942

Graphical Abstract


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