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Microbiology and Biotechnology Letters

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Environmental Microbiology (EM)  |  Microbial Ecology and Diversity

Microbiol. Biotechnol. Lett. 2021; 49(3): 367-373

https://doi.org/10.48022/mbl.2102.02005

Received: February 13, 2021; Revised: April 7, 2021; Accepted: April 8, 2021

Ambient Air Waste Sorting Facilities Could Be a Source of Antibiotic Resistant Bacteria

Ana Calheiros1, Joana Santos1,2, Carla Ramos1, Marta Vasconcelos2,3, and Paulo Fernandes1,2*

1Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347, Viana do Castelo, Portugal 2CISAS, Escola Superior de Tecnologia e Gestão, Instituto Politécnico de Viana do Castelo, Rua Escola Industrial e Comercial de Nun’Álvares, 4900-347, Viana do Castelo, Portugal 3Instituto Politécnico de Coimbra, ESTESC–Coimbra Health School, Saúde Ambiental, Coimbra, Portugal

Correspondence to :
Paulo Fernandes,     paulof@estg.ipvc.pt

The antimicrobial resistance of Staphylococcus spp. and Gram negative strains present in air samples from waste sorting facilities was assessed. Phenotypic studies have revealed a high percentage of strains of Staphylococcus spp. resistant to methicillin. Genotypically and by RT-PCR, it was found that the mecA gene usually associated with methicillin resistance was present in 8% of the Staphylococcus strains isolated. About 30% of the Gram negative strains from the same samples also displayed resistance to meropenem and 79% of these were resistant to multiple antibiotics from different classes, namely cephalosporins and β- lactams. The results suggest that in professional activities with high levels of exposure to biological agents, the quantification and identification of the microbial flora in the work environment, with the determination of the presence of potential agents displaying multi-resistances is of relevance to the risk assessment. The personal protection of workers is particularly important relevance in these cases, since many of the strains that exhibit multi-resistance are potential opportunistic agents.

Keywords: Staphylococcus, Enterobacteriaceae, biological risk, antimicrobial resistance

Graphical Abstract


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