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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2021; 49(3): 283-288

https://doi.org/10.48022/mbl.2102.02009

Received: February 24, 2021; Revised: March 30, 2021; Accepted: April 1, 2021

굴피나무 잎 추출물의 위암세포에 대한 세포사멸 유도 효과

Induction of Apoptosis in AGS Human Gastric Cancer Cells by Platycarya strobilacea Leaf Extract

Hyeong-Seon Lee*

Department of Biomedical Laboratory Science, Jungwon University, Goesan 28024, South Korea

Correspondence to :
Hyeong-Seon Lee,      biohslee@jwu.ac.kr

This study investigated the anticancer activity of methanol extract from Platycarya strobilacea leaf in AGS human gastric cancer cells. We determined the cell viability effect of P. strobilacea using MTS assay. Apoptosis induction and cell cycle arrest were confirmed by fluorescein isothiocyanate and propidium iodide staining using cellometer K2. The mRNA expression levels of the Bcl-2 family were confirmed by reverse transcription-polymerase chain reaction. The cell viability was decreased in a dose-dependent manner treated with different concentrations of P. strobilacea. Total, early, and late apoptotic cells were dramatically increased, and the cell cycle was arrested at the sub-G1 phase. The mRNA expressions of Bcl-2 and Bcl-xL were reduced, whereas pro-apoptotic factors, Bax and Bak, were increased in a dose-dependent manner. These results suggested that P. strobilacea leaf extract induced significant apoptotic activity through an intrinsic mitochondria pathway.

Keywords: Platycrya strobilacea, AGS human gastric cancer cells, Bcl-2 family, apoptosis

Graphical Abstract


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