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Microbiology and Biotechnology Letters

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Fermentation Microbiology (FM)  |  Fermentation Technology

Microbiol. Biotechnol. Lett. 2021; 49(2): 210-216

https://doi.org/10.48022/mbl.2102.02008

Received: February 20, 2021; Revised: March 23, 2021; Accepted: March 24, 2021

CRISPR/CAS9을 이용하여 lipid elongation gene의 과발현을 통한 효모의 에탄올 발효능 개선

Enhancement of Ethanol Productivity with Saccharomyces cerevisiae by Overexpression of Lipid Elongation Gene Using CRISPR/CAS9

JinA Kim and Gwi-Taek Jeong*

Department of Biotechnology, Pukyong National University, Busan 48513, Republic of Korea

Correspondence to :
Gwi-Taek Jeong,      gtjeong@pknu.ac.kr

This study aimed to enhance ethanol productivity of Saccharomyces cerevisiae through genome editing using CRISPR/CAS9. To increase ethanol productivity, ACC1, ELO1, and OLE1 were overexpressed in S. cerevisiae using the CRISPR/CAS9 system. The strains overexpressing ACC1, ELO1, and OLE1 survived up to 24 h in YPD medium supplemented with 18% ethanol. Moreover, the ethanol yields in strains overexpressing ACC1 (428.18 mg ethanol/g glucose), ELO1 (416.15 mg ethanol/g glucose), and OLE1 (430.55 mg ethanol/g glucose) were higher than those in the control strains (400.26 mg ethanol/g glucose). In conclusion, the overexpression of these genes increased the viability of S. cerevisiae at high ethanol concentrations and the ethanol productivity without suppressing glucose consumption.

Keywords: Ethanol fermentation, ethanol tolerance, lipid elongation gene, ethanol productivity

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