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Molecular and Cellular Microbiology (MCM)  |  Microbial Genetics, Physiology and Metabolism

Microbiol. Biotechnol. Lett. 2021; 49(2): 239-248

https://doi.org/10.48022/mbl.2008.08012

Received: August 20, 2020; Revised: March 16, 2021; Accepted: March 16, 2021

Genotyping and Molecular Characterization of Carbapenem-resistant Acinetobacter baumannii Strains Isolated from Intensive Care Unit Patients

Rania Abozahra1*, Sarah M. Abdelhamid1, Amel G. Elsheredy2, Kawther E. Abdulwahab1, and Kholoud Baraka1

1Department of Microbiology and Immunology, Faculty of Pharmacy, Damanhour University, Damanhour, Egypt 2Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt

Correspondence to :
Rania Abozahra,     rania_abozahra@yahoo.com

The emergence of multidrug-resistant Acinetobacter baumannii has partly increased treatment failure and patient mortality. Class D β-lactamases is an important mechanism of resistance to beta-lactam antibiotics in this species. This study aimed to investigate the relationship between the presence oxacillinase gene and genetic fingerprints of A. baumannii isolates from the intensive care unit of an Egyptian tertiary care hospital. One hundred and twenty A. baumannii clinical isolates were collected. Multiplex PCR was performed to detect genes encoding oxacillinases (OXA-23, OXA-24, OXA-51, OXA-58 and OXA-143). Molecular typing of all collected isolates was performed using random amplified polymorphic DNA (RAPD)-PCR assay. Out of 120 examined isolates, 92, 88 and 84% were resistant to ertapenem, imipenem and meropenem, respectively. The species-specific, commonly present OXA-51 gene was found in all isolates while OXA-23 showed a high prevalence of 88% of isolates. OXA-24 and OXA-143 genes were detected in 3% and 1% of isolates, respectively. No OXA-58 gene was detected. Five clusters consisting of 19 genotypes were detected using RAPDPCR. Genotype A was the most prevalent, it was observed in 62% of the isolates followed by genotype B (12%). These results revealed that genotypes A and B are common in the hospital. Results also demonstrate that RAPD-PCR is a rapid and reliable method for studying the clonal similarity among A. baumannii isolated from different clinical specimens.

Keywords: Acinetobacter baumannii, carbapenem resistance, oxacillinase genes, genotyping

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