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Microbiology and Biotechnology Letters

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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2021; 49(1): 57-64

https://doi.org/10.48022/mbl.2102.02006

Received: February 18, 2021; Revised: March 13, 2021; Accepted: March 15, 2021

헴철이 풍부한 영양원이 혐기성 세균의 생장과 생존에 미치는 영향: 락토바실러스 가세리 모델연구

Effect of Heme-rich Nutrient on Anaerobic Bacterial Growth and Survival: A Model Study on Lactobacillus gasseri

Seungki Lee# and Pil Kim*

Department of Biotechnology, The Catholic University of Korea, Bucheon 14662, Republic of Korea #Current address: SL: Dibiome Co., Anyang, Gyeonggi 14102, Korea

Correspondence to :
Pil Kim, 
kimp@catholic.ac.kr

Lactic acid bacteria (LAB), belonging to the Firmicutes phylum, lack heme biosynthesis and, thus, are characterized as fermentative and catalase-negative organisms. To verify the hypothesis that heme-rich-nutrients might compensate the heme-biosynthesis incapability of non-respiratory LAB in animal gut, a heme-richnutrient was fed to a dog and its fecal microbiome was analyzed. Firmicutes abundance in the feces from the heme-rich-nutrient-fed dog was 99%, compared to 92% in the control dog. To clarify the reason of increased Firmicutes abundance in the feces from the heme-rich-nutrient-fed dog, Lacobacillus gasseri were used as model anerobic LAB to study a purified heme (hemin). The anaerobic growth of L. gasseri in the medium with 25 μM hemin supplementation was faster than that in the medium without hemin, while the growth in the 50 μM hemin-supplemented medium did not vary. Cellular activities of the cytochrome bd complex were 1.55 ± 0.19, 2.11 ± 0.14, and 2.20 ± 0.08 U/gcell in the cells from 0, 25, and 50 μM hemin-supplemented medium, while intracellular ATP concentrations were 7.90 ± 1.12, 11.95 ± 0.68, and 12.56 ± 0.58 μmolATP/gcell, respectively. The ROS-scavenging activities of the L. gasseri cytosol from 25 μM and 50 μM hemin-supplemented medium were 68% and 82% greater than those of the cytosol from no hemin supplemented-medium, respectively. These findings indicate that external hemin could compensate the heme-biosynthesis incapability of L. gasseri by increasing the cytosolic ROS-scavenging and extra ATP generation, possibly through increasing the electron transfer. Increase in the number of anaerobic bacteria in heme-rich-nutrient-fed animal gut is discussed based on the results.

Keywords: Heme-rich-nutrient, lactic acid bacteria, anaerobic bacteria, gut microbiome, dog

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