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Microbiology and Biotechnology Letters

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Environmental Microbiology (EM)  |  Microbial Ecology and Bioremediation

Microbiol. Biotechnol. Lett. 2021; 49(1): 1-8

https://doi.org//10.48022/mbl.2010.10011

Received: October 19, 2020; Accepted: November 2, 2020

Isolation of Novel Strains of Lactobacillus gasseri EJL and Bifidobacterium breve JTL from Breast Milk and Infant Feces: A Longitudinal Study of a Mother-infant Pair

Heetae Lee1, Chong-Kil Lee2 and Kyungjae Kim1*

1College of Pharmacy, Sahmyook University, Seoul 01795, Republic of Korea 2College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea

Correspondence to :
Kyungjae Kim,
kimkj@syu.ac.kr

Human breast milk is a potential source of bacteria for the development of the intestinal microbiota of infants. Several species within the genera Lactobacillus and Bifidobacterium were demonstrated to shape the gut microbiota of infants. In this study, the bacterial diversity was investigated in the breast milk and feces of a mother-infant pair, and probiotic candidates were identified. Importantly, the novel L. gasseri EJL and B. breve JTL strains were isolated from breast milk and infant feces samples, respectively; their completed genome was resolved using de novo sequencing. In addition, the bacterial composition in the infant’s feces at 1 week revealed the prevalence of Bifidobacterium and Streptococcus; a higher diversity was observed after 3 weeks. In particular, the abundance of Akkermansia was sharply increased at 7 weeks, further increasing thereafter, up to 15 weeks. Our results suggest that human breast milk and infant’s feces are a source of probiotic candidates.

Keywords: Human breast milk, Lactobacillus gasseri, Bifidobacterium breve, gut microbiota, probiotics

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