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Food Microbiology (FM)  |  Bioactive Compounds or Metabolites: Function and Application

Microbiol. Biotechnol. Lett. 2021; 49(1): 32-38

https://doi.org/10.48022/mbl.2010.10001

Received: October 6, 2020; Accepted: November 28, 2020

Screening of Myxobacteria Carrying Tubulysin Biosynthetic Genes

Hyesook Hyun1, Juo Choi1, 2, Daun Kang2, Yungpil Kim2, Pilgoo Lee 2, Gregory Chung 2 and Kyungyun Cho1*

1Department of Biotechnology, Hoseo University, Asan 31499, Republic of Korea 2MECOX CureMed Co., Seoul 06744, Republic of Korea

Correspondence to :
Kyungyun Cho,
kycho@hoseo.edu

Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B.

Keywords: Archangium gephyra, myxobacteria, secondary metabolite, tubulysin

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