Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Microbial Biotechnology (MB)  |  Cell Culture and Biomedical Engineering

Microbiol. Biotechnol. Lett. 2021; 49(2): 167-173

https://doi.org/10.48022/mbl.2012.12001

Received: December 4, 2020; Revised: February 16, 2021; Accepted: February 26, 2021

A Study on the Tyrosinase Inhibitory and Antioxidant Effect of Microalgae Extracts

Keunho Ji1, Yeeum Kim2 and Young Tae Kim2*

1Basic Science Research Institute, 2Deptartment of Microbiology, Pukyong National University, Busan 48513, Republic of Korea

Correspondence to :
Young Tae Kim,     ytkim@pknu.ac.kr

Abstract

Go to

Reactive oxygen species (ROS) disrupt the cellular redox balance, exert cytotoxic effects, and consequently promote the development of various diseases in humans. Previous studies have reported that antioxidants counteract the adverse effects of ROS. Several studies examine the whitening effects of various agents based on their ability to inhibit tyrosinase activity. Tyrosinase is a critical enzyme involved in the synthesis of melanin, which protects the skin against radiation. Various agents exhibiting antioxidant and tyrosinase inhibitory activities have been synthesized. However, these synthetic drugs are associated with toxicity, decreased safety, and poor skin penetration in vivo, which has limited the clinical application of synthetic drugs. This study examined the antioxidant and tyrosinase inhibitory activities of some microalgae. The methanol, dichloromethane, and ethyl acetate extracts of four microalgal species (Tetraselmis tetrathele, Dunaliella tertiolecta, Platymonas sp., and Chaetoceros simplex) were prepared. The physiological and whitening effects of microalgal extracts were investigated by measuring the antioxidant and tyrosinase inhibitory activities. The ethyl acetate extract of D. tertiolecta exhibited the highest antioxidant and tyrosinase inhibitory activities. Future studies must focus on examining the whitening effects of microalgae on cell lines to facilitate the development of microalga-based therapeutics for skin diseases, functional health foods, and whitening agents. Thus, microalgae have potential applications in the pharmaceutical, food, and cosmetic industries.

Keywords:

Antioxidant, cosmeceutical, Dunaliella, microalgae, tyrosinase inhibition

References

Go to
  1. Koh YJ, Cha DS, Choi HD, Park YK, Choi IW. 2008. Hot water extraction optimization of dandelion leaves to increase antioxidant activity. Korean J. Food Sci. Technol. 40: 283-289.
  2. Seo BY, Choi MJ, Choi EA, Park E. 2014. In vitro antioxidant effects of Sarijang. J. Korean Soc. Food Sic. Nut. 43: 618-623.
  3. Choi SY, Kim JH, Lee J, Cho EJ. 2017. Protective effect of Acer okamotoanum from oxidative stress in C6 glial cells. J. Appl. Biol. Chem. 60: 141-147.
  4. Kwak JY, Seok JK, Suh HJ, Hong SS, Kim DS, Boo YC. 2016. Antimelanogenic effects of luteolin 7‐sulfate isolated from Phyllospadix iwatensis Makino. Trans. Res. 50: 501-511.
    Pubmed
  5. Ji K, Cho YS, Kim YT. 2018. Tyrosinase inhibitory and anti-oxidative effects of lactic acid bacteria isolated from dairy cow feces. Probiotics Antimicrob. Proteins 10: 43-55.
    Pubmed
  6. Yoon NY, Eom TK, Kim MM, Kim SK. 2009. Inhibitory effect of phlorotannins isolated from Ecklonia cava on mushroom tyrosinase activity and melanin formation in mouse B16F10 melanoma cells. J. Agric. Food Chem. 57: 4124-4129.
    Pubmed
  7. Cha SH, Ko SC, Kim D, Jeon YJ. 2011. Screening of marine algae for potential tyrosinase inhibitor: those inhibitors reduced tyrosinase activity and melanin synthesis in zebrafish. J. Dermatol. 38:354-363.
    Pubmed
  8. Pereira H, Custodio L, Rodrigues MJ, Sousa Bd, Oliveira M, Barreira L, et al. 2015. Biological activities and chemical composition of methanolic extracts of selected autochthonous microalgae strains from the Red Sea. Mar. Drugs 13: 3531-3549.
    Pubmed KoreaMed
  9. Andrade LM, Andrade CJ, Dias M, Nascimento CAO, Mendes MA. 2018. Chlorella and spirulina microalgae as sources of functional doods, nutraceuticals and food supplements; an overview. MOJ Food Process Technol. 6: 45-58.
  10. Rizwan M, Mujtaba G, Memon SA, Lee K, Rashid N. 2018. Exploring the potential of microalgae for new biotechnology applications and beyond: a review. Renew. Sustain. Energy Rev. 92: 394404.
  11. Wang T, Jonsdottir R, Ólafsdóttir G. 2009. Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds. Food Chem. 116: 240-248.
  12. Morowvat MH, Ghasemi Y. 2016. Evaluation of antioxidant properties of some naturally isolated microalgae: Identification and characterization of the most efficient strain. Biocata. Agric. Biotechnol. 8: 263-269.
  13. Ben-Amotz A. 1995. New mode of Dunaliella biotechnology:two-phase growth for β-carotene production. J. Appl. Phycol. 7: 65-68.
  14. Hosseini Tafreshi A, Shariati M. 2009. Dunaliella biotechnology:methods and applications. J. Appl. Microbiol. 107: 14-35.
    Pubmed
  15. Borowitzka MA. 1995. Microalgae as sources of pharmaceuticals and other biologically active compounds. J. Appl. Phycol. 7: 3-15.
  16. Villar R, Laguna MR, Calleja JM, Cadavid I. 1992. Effects of Phaeodactylum tricornutum and Dunaliella tertiolecta extracts on the central nervous system. Planta Med. 58: 405-409.
    Pubmed
  17. Chang T, Ohta S, Ikegami N, Miyata H, Kashimoto T, Kondo M. 1993. Antibiotic substances produced by a marine green alga, Dunaliella primolecta. Bioresour. Technol. 44: 149-153.
  18. Karthikeyan P, Manimaran K, Sampathkumar P, Jaikumar M, Robin RS, Saravanakumar C, et al. 2013. In vitro antioxidant activity of marine diatoms. J. Environ. Sci. Toxicol. Food Technol. 5: 32-37.
  19. Farahin AW, Yusoff FM, Nagao N, Basri M, Shariff M. 2016. Pheno lic content and antioxidant activity of Tetraselmis tetrathele (West) Butcher 1959 cultured in annular photobioreactor. J. Environ. Biol. 37: 631-639.
  20. Abd El-Baky HH, Hussein MM, El-Baroty GS. 2008. Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant irrigated with sea water. Afr. J. Biochem. Res. 2: 151-164.
  21. Yoo C, Kim CJ, Choi GG, Ahn CY, Choi JS, Oh HM. 2009. A mutant Arthrospira platensis M20CJK3 showing enhanced growth rate and floatation activity. Korean J. Microbiol. 45: 268-274.
  22. Cordero BF, Obraztsova I, Couso I, Leon R, Vargas MA, Rodriguez H. 2011. Enhancement of lutein production in Chlorella sorokiniana (Chorophyta) by improvement of culture conditions and random mutagenesis. Mar. Drugs 9: 1607-1624.
    Pubmed KoreaMed

Article Tools

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.