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Microbiology and Biotechnology Letters

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Biocatalysis and Bioprocess Engineering  |  Enzyme, Protein Engineering, and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2020; 48(1): 32-37

https://doi.org/10.4014/mbl.1909.09008

Received: September 17, 2019; Accepted: October 23, 2019

효모에서 agaro-bioethanol 생산을 위한 단일화된 당화 발효 시스템의 개량

Improvement of a Unified Saccharification and Fermentation System for Agaro-bioethanol Production in Yeast

So-Eun Lee and Yeon-Hee Kim *

Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering, College of Engineering, Dong-Eui University

We improved on a unified saccharification and fermentation (USF) system for the direct production of ethanol from agarose by increasing total agarase activity. The pGMFα-NGH plasmid harboring the NABH558 gene encoding neoagarobiose hydrolase and the AGAG1 and AGAH71 genes encoding β-agarase was constructed and used to transform Saccharomyces cerevisiae 2805. NABH558 gene transcription level was increased and total agarase activity was increased by 25 to 40% by placing the NABH558 gene expression cassette upstream of the other gene expression cassettes. In the 2805/pGMFα-NGH transformant, three secretory agarases were produced that efficiently degraded agarose to galactose, 3,6-anhydro-L-galactose (AHG), neoagarobiose, and neoagarohexaose. During the united cultivation process, a maximum of 2.36 g/l ethanol from 10 g/l agarose was produced over 120 h.

Keywords: Unified enzymatic saccharification and fermentation (USF) system, &beta,-agarase, neoagarobiose hydrolase, bioethanol, recombinant yeast

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