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Microbiology and Biotechnology Letters

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Biocatalysis and Bioprocess Engineering  |  Enzyme, Protein Engineering, and Metabolic Engineering

Microbiol. Biotechnol. Lett. 2017; 45(1): 43-50

https://doi.org/10.4014/mbl.1611.11002

Received: November 8, 2016; Accepted: January 10, 2017

제주도 토양에서 분리한 xylanase 생산 균주 Streptomyces glaucescens subsp. WJ-1의 동정 및 효소의 생화학적 특성 연구

Identification and Biochemical Characterization of Xylanase-producing Streptomyces glaucescens subsp. WJ-1 Isolated from Soil in Jeju Island, Korea

Da Som Kim 1, Sung Cheol Jung 2, Chang Hwan Bae 1 and Won-Jae Chi 1*

1Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon 22689, Republic of Korea, 2Warm-temperature and Subtropical Forest Research Center, Korea Forest Research Institute, Jeju 63582, Republic of Korea

A xylan-degrading bacterium (strain WJ-1) was isolated from soil collected from Jeju Island, Republic of Korea. Strain WJ-1 was characterized as a gram-positive, aerobic, and spore-forming bacterium. The predominant fatty acid in this bacterium was anteiso-C15:0 (42.99%). A similarity search based on 16S rRNA gene sequences suggested that the strain belonged to the genus Streptomyces. Further, strain WJ-1 shared the highest sequence similarity with the type strains Streptomyces spinoveruucosus NBRC 14228, S. minutiscleroticus NBRC 13000, and S. glaucescens NBRC 12774. Together, they formed a coherent cluster in a phylogenetic tree based on the neighbor-joining algorithm. The DNA G+C content of strain WJ-1 was 74.7 mol%. The level of DNA–DNA relatedness between strain WJ-1 and the closest related species S. glaucescens NBRC 12774 was 85.7%. DNA–DNA hybridization, 16S rRNA gene sequence similarity, and the phenotypic and chemotaxonomic characteristics suggest that strain WJ-1 constitutes a novel subspecies of S. glaucescens. Thus, the strain was designated as S. glaucescens subsp. WJ-1 (Korean Agricultural Culture Collection [KACC] accession number 92086). Additionally, strain WJ-1 secreted thermostable endo-type xylanases that converted xylan to xylooligosaccharides such as xylotriose and xylotetraose. The enzymes exhibited optimal activity at pH 7.0 and 55℃.

Keywords: xylanase, Streptomyces glaucescen, identification, characterization, phylogenetic analysis

  1. Subramaniyan S, Prema P. 2002. Biotechnology of microbial xylanases: Enzyology, molecular biology, and application. Crit. Rev. Biotechnol. 22: 33-64.
    Pubmed CrossRef
  2. Beg QK, Kapoor M, Mahajan L, Hoondal GS. 2001. Microbial xylanases and their industrial applications: a review. Appl. Microbiol. Biotechnol. 56: 326-338.
    Pubmed CrossRef
  3. Bajaj BK, Singh NP. 2010. Production of xylanase from an alkalitolerant Streptomyces sp. 7b under solid-state fermentation, its purification, and characterization. Appl. Biochem. Biotechnol. 162: 1804-1818.
    Pubmed CrossRef
  4. Collins T, Gerday C, Feller G. 2005. Xylanases, xylanase families and extremophile xlanases. 2005. FEMS Microbiol. Rev. 29: 3-23.
    Pubmed CrossRef
  5. Kieser H, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA. 2000. Practical Streptomyces genetics. The John Innes Foundation, Norwich, United Kingdom.
    KoreaMed
  6. Baker GC, Smith JJ, Cowan DA. 2003. Review and re-analysis of domain-specific 16S primers. J. Microbiol. Methods 55: 541-555.
    Pubmed CrossRef
  7. Chun J, Lee JH, Jung YY, Kim MJ, Kim SI, Kim BK, et al. 2007. ExTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57: 2259-2261.
    Pubmed CrossRef
  8. Thomson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
    CrossRef
  9. Hall TA. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95-98.
  10. Felsenstein J. 1993. PHYLIP (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seatle, USA.
    KoreaMed
  11. Saitou N, Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
    Pubmed
  12. Kluge AG, Farris FS. 1969. Quantative phyletics and the evolution of anurans. Syst. Zool. 18: 1-32.
    CrossRef
  13. Kimura M. 1983. The Neutral Theory of Molecular Evolution. Cambridge University Press, Cambridge, UK.
    CrossRef
  14. Miller L, Berger T. 1985. Bacterial identification by gas chromatography of whole cell fatty acid. Hewlett-Packard Application note. pp. 228-241.
  15. Sasser M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Inc., Newark, DE, USA.
  16. Mesbah M, Premachandran U, Whitman WB. 1989. Precise measurement of the G+C content of deoxyribonucleic acid by highperformance liquid chromatography. Int. J. Syst. Bacteriol. 39:159-167.
    CrossRef
  17. Komagata K, Suzuki K. 1987. Lipid and cell-wall analysis in bacterial systematic. Methods Microbiol. 19: 161-207.
    CrossRef
  18. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426-428.
    CrossRef
  19. Van Trappen S, Tan TL, Yang J, Mergaert J, Swings J. 2004. Altermonas stellipolaris sp. nov., a novel, budding, prosthecate bacterium from Antarctic seas, and emended description of the genus Altermonas. Int. J. Syst. Evol. Microbiol. 54: 1157-1163.
    Pubmed CrossRef
  20. Pridham, TG, Hesseltine CW, Benedict RG. 1958. A guide for the classification of Streptomycetes according to selected groups;placement of strains in morphological sections. Appl. Microbiol. 6: 52-79.
    Pubmed KoreaMed
  21. Virupakshi S, Babu KG, Gaikwad SR, Naik GR. 2005. Production of a xylanolytic enzyme by a thermoalkaliphilic Bacillus sp. JB-99 in solid state fermentation. Proc. Biochem. 40: 431-435.
    CrossRef
  22. Miyazono K, Tabei N, Morita S, Ohnishi Y, Horinouchi S, Tanokura M. 2012. Substrate recognition mechanism and substratedependent conformational changes of an ROK family glucokinase from Streptomyces griseus. J. Bacteriol. 194: 607-616.
    Pubmed KoreaMed CrossRef
  23. Angell S, Lewis CG, Buttner MJ, Bibb MJ. 1994. Glucose repression in Streptomyces coelicolor A3(2): a likely regulatory role for glucose kinase. Mol. Gen. Genet. 244: 135-143.
    Pubmed CrossRef
  24. Georis J, Giannotta F, Buyl ED, Granier B, Frere JM. 2000. Purification and properties of three endo-β-1,4-xylanases produced by Streptomyces sp. strain S38 which differ in their ability to enhance the bleaching of kraft pulps. Enz. Microbial. Technol. 26:178-186.
    CrossRef
  25. Ninawe S, Kapoor M, Kuhad RC. 2008. Purification and characterization of extracellular xylanase from Streptomyces cyaneus SN32. Bioresour. Technol. 99: 1252-1258.
    Pubmed CrossRef
  26. Wang SL, Yen YH, Shih IL, Chang AC, Chang WT, Wu WC, et al. 2003. Production of xylanases from rice bran by Streptomyces actuosus A-151. Enz. Microbial Technol. 33: 917-925.
    CrossRef
  27. Zhang J, Matti SA, Terhi P, Ming T, Maija T, Liisa V. 2011. Thermostable recombinant xylanases from Nonomuraea flexuosa and Thermoascus aurantiacus show distinct properties in the hydrolysis of xylans and pretreated wheat straw. Biotechnol. Biofuels 4:12-25.
    Pubmed KoreaMed CrossRef

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