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Microbiology and Biotechnology Letters

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Microbiol. Biotechnol. Lett. 2010; 38(4): 399-404

수소생산균 Enterobacter sp. ES392의 분리 및 배양조건

Isolation and Culture Conditions of Hydrogen Producing Bacterium Enterobacter sp. ES392

Sung-Jong Jeon and Eon-Seok Lee

Department of Biomaterial Control (Brain Korea 21 program), Dong-Eui University, 1Department of Biomaterial Control (Brain Korea 21 program), Dong-Eui University

Abstract

A hydrogen-producing bacterium (strain ES392) was isolated from pond water located in the Dong-Eui University, Busan, Korea. The cell was long-rod type ($1.4;{mu}m$) of about ($0.6;{mu}m$) in diameter, and not formed flagellum and spore. Phylogenetic analysis based on the 16S rRNA sequence and biochemical studies indicated that ES392 belonged to the genus Enterobacter sp. The optimum pH and temperature for hydrogen production was 7.5 and $35^{circ}C$, respectively. The optimization of medium compositions which maximize hydrogen production from Enterobacter sp. ES392 was determined. As a result, the maximum hydrogen production was obtained under the conditions of 4% (w/v) sucrose, 0.5% (w/v) yeast extract and 50 mM potassium phosphate buffer (pH 7.5). Under batch culture conditions, the maximal hydrogen production and yield were obtained as 3481 mL/L and 1.33 mol/mol sucrose, respectively.

Keywords: Hydrogen production, Enterobacter, medium composition

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