Genome Report | Genome Report
Microbiol. Biotechnol. Lett. 2023; 51(4): 538-541
https://doi.org/10.48022/mbl.2311.11003
Lin He1, Huan Luo1, Mi-Hyun Lee2, and Jun Myoung Yu1*
1Department of Applied Biology, Chungnam National University, Daejeon 34134, Republic of Korea
2Crop Protection Division, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea
Correspondence to :
Jun Myoung Yu, junmyu@gmail.com
In this study, we present the draft genome of Aureobasidium pullulans strain MHAU2101, which is the first strain to effectively control fire blight caused by Erwinia amylovora in Korea. The genome of strain MHAU2101 was composed of 28,669,322 base pairs, with a C+G content of 50.4%. The assembly comprised 17 contigs and had 99.22% completeness. The results of this study will be a valuable resource for future research on the biocontrol mechanism of A. pullulans strain MHAU2101.
Keywords: Aureobasidium pullulans, biocontrol, fire blight, PacBio, Illumina, draft genome
In this study, we sequenced the genome of
In total, 140,079 HiFi reads were obtained from PacBio sequencing, with a HiFi N50 value of 8,264 bp (half of all bases resided in reads of this size or longer), and an average read length of 7,301 bp, consisting of 1,022,750,246 HiFi bases. Illumina sequencing generated approximately 2,022.9 Mb of raw data, and 1,364.2 Mb of clean data were retained after filtering.
The assembled draft genome was 28,669,322 bp in size, comprising 17 contigs and 50.4% G+C content (Fig. 1). The N50 of the genome was 2,213,608 bp. The length of the 17 contigs ranged from 18,956 bp to 4,494,408 bp, and the average length of a contig was 1,686,430 bp. The BUSCO results indicated that the genome assembly displayed a high overall completeness of 99.22%; only 0.78% of BUSCOs were present as fragments, and none were missing in the analysis. The genome contained 20,765 introns and 32,338 exons in total. Moreover, 11,573 protein-coding genes, 313 transfer RNAs, and 70 ribosomal RNA coding sequences were predicted. The features of the strain MHAU2101 genome are shown in Table 1. A total of 6,043 proteins were assigned to the GO database, 7,664 proteins were assigned to the InterPro database, 7,864 proteins were assigned to the Pfam database, 3,511 proteins were assigned to the CDD database, 878 proteins were assigned to the TIGRFAM database, and 11,054 proteins were assigned to the EggNOG database.
Table 1 . Genome statistics of
Features Strain | MHAU2101 |
---|---|
PacBio platform | |
Number of read | 140,079 |
Total length of reads | 1,022,750,246 bp |
N50 length | 8,264 bp |
Average read length | 7,301 bp |
Illumina platform | |
Raw data of read | 13,396,436 |
Clean data of read | 9,037,346 |
Clean data Q20 | 99.32% |
Clean data Q30 | 96.78% |
Clean data GC | 49.59% |
Genome assembly | |
Contig | 17 |
Contig size | 28,669,322 bp |
N50 length | 2,213,608 bp |
GC content | 50.4% |
Number of exons | 32, 338 |
Number of introns | 20,765 |
Putative protein-coding genes | 11,573 |
Number of tRNA | 313 |
Number of rRNA | 70 |
BUSCOs | |
Complete and single-copy BUSCOs | 253 (99.22%) |
Complete and duplicated BUSCOs | 0 (0.00%) |
Fragmented BUSCOs | 2 (0.78%) |
Missing BUSCOs | 0 (0.00%) |
Total BUSCO groups searched | 255 (100%) |
In summary, we present a high-quality draft genome assembly of
The draft genome sequence of
This work was supported by a grant from the Agenda program (PJ015594) of the Rural Development Administration, Republic of Korea.
The authors have no financial conflicts of interest to declare.
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