Food, Environment, and Other Topics in Biotechnology
Microbiol. Biotechnol. Lett. 2014; 42(4): 354-360
https://doi.org/10.4014/kjmb.1407.07009
Jung Ae Park 1, Kyong-Suk Jin 1, Hyun Ju Kwon 1, 2 and Byung Woo Kim 1, 2*
1Blue-Bio Industry Regional Innovation Center, Dong-Eui University, Busan 614-714, Republic of Korea, 2Department of Life Science and Biotechnology, College of Natural Science and Human Ecology, Dong-Eui University, Busan 614-714, Republic of Korea
In this study, the anti-obesity activity of Smilax china methanol extract (SCME) was evaluated using a pancreatic lipase enzyme inhibition assay, and a cell culture model system. Results indicated that, SCME effectively inhibited pancreatic lipase enzyme activity in a dose-dependent manner. Furthermore, SCME significantly suppressed insulin, dexamethasone, 3-isobutyl-1-methylxanthine- induced adipocyte differentiation, lipid accumulation, and triglyceride contents on 3T3-L1 preadipocytes, in a dosedependent manner. The anti-adipogenic effect was modulated by cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/ enhancer binding proteins (C/EBP) α, C/EBPβ, and the peroxisome proliferator-activated receptor γ gene and protein expressions. Moreover, SCME triggered lipolysis effects dose-dependently on adipocyte. Taken together, these results provide an important new insight into SCME, indicating that it possesses anti-obesity activity through pancreatic lipase inhibition, anti-adipogenic and lipolysis effects. SCME may therefore be utilized as a promising source in the field of nutraceuticals. The identification of active compounds that confer the anti-obesity activities of SCME may be a logical next step.
Keywords: Smilax china methanol extract, anti-obesity activity, lipase inhibition activity, anti-adipogenic and lipolysis effects
Jung Ae Park , Kyong-Suk Jin , Hyun Ju Kwon and Byung Woo Kim
Microbiol. Biotechnol. Lett. 2015; 43(4): 336-342 https://doi.org/10.4014/mbl.1509.09002Jung Ae Park , Kyong-Suk Jin , Ji Young Lee , Hyun Ju Kwon and Byung Woo Kim
Microbiol. Biotechnol. Lett. 2014; 42(3): 249-257 https://doi.org/10.4014/kjmb.1403.03001Jung Ae Park , Kyong-Suk Jin , Ji Young Lee , Hyun Ju Kwon and Byung Woo Kim
Microbiol. Biotechnol. Lett. 2013; 41(3): 341-349 https://doi.org/10.4014/kjmb.1306.06002