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Microbiology and Biotechnology Letters


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Molecular and Cell biology, and Microbial Isolation

Microbiol. Biotechnol. Lett. 2012; 40(2): 98-103

Received: May 10, 2012; Accepted: June 13, 2012

메주로부터 지질분해 효소 생산 균주의 분리 및 배양학적 특성

The Isolation and Culture Characterization of a Lipolytic Enzyme Producing Strain from Meju

Hye Ju Yun 1, 2, You Jung Lee 1, Soo-Hwan Yeo 1, Hye Sun Choi 1, Hye Young Park 1, Heui-Dong Park 2 and Seong Yeol Baek 1*

1Fermentation & Food Processing Division, Department of Agro-food Resource, NAAS, RDA, Suwon, Korea, 2Department of Food Science & Technology, Agro Biotechnology Education Center, Kyungpook National University, Daegu, Korea

For screening of useful enzymes producing microorganisms from Meju, we isolated high lipase producing strains and their lipolytic enzyme activities were then tested. The lipolytic enzyme activities of isolated microorganisms were therefore tested on the Y124 strain. The gene sequence analysis of ITS from Y124 strain revealed Yarrowia lipolytica. Lipase production by the Y124 strain was studied in media containing various carbon sources. The Y124 strain drastically increased lipolytic enzyme activity in YPO media containing olive oil, as well as in YPDO media containing both olive oil and glucose. Maximal lipase production was achieved in YPD (yeast extract-peptone-D-glucose) media containing 0.7% olive oil when cultured at 30oC for 8 hrs. The lipase produced from the Y124 strain showed the highest activity in p-NPO (p-nitrophenyl octanoate (C8)), amongst the various p-nitrophenyl esters.

Keywords: Yarrowia lipolytica, 16S rDNA, Lipase activity, carbon source

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