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Microbiology and Biotechnology Letters


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Microbiol. Biotechnol. Lett. 2020; 48(4): 569-573

Received: July 26, 2020; Accepted: August 28, 2020

Increasing of Macrophage Migration Inhibitory Factor Expression in Human Patients Infected with Virulent Brucella in Iraq

Hasan Raheem Khudhur1*, Abbas Ali Menshed2 and Ahmed Abbas Hasan3

¹Department of Medical Microbiology, AL-Muthanna University, 66001, Iraq 2Ministry of Education, ³College of Health and Medical Techniques, Al-Furat Al-Awsat Technical University, 31003, Iraq

Correspondence to :
Hasan  Raheem Khudhur,

Brucellosis is a zoonotic disease caused by Brucella infections and humans usually contract this disease from close contact with infected animals or their products, usually via the ingestion of cheese or crude milk. Macrophage migration inhibitory factor (MIF) and Pro- and anti-inflammatory cytokines play an important role in susceptibility/resistance and the immunopathogenesis of Brucella infection. These cytokines are crucial factors in the initiation and progression of protective immunity against Brucella infection but the role of MIF has not been well studied in the human response to intracellular microbes. This study was designed to investigate the effect of MIF expression on Brucella susceptibility. A total of 85 positive rose Bengal tests and 24 samples from healthy individuals were collected for this study and subjected to polymerase chain reaction assays (PCR) of the bcsp31 diagnostic gene. MIF concentrations were evaluated using Enzyme-Linked immunosorbent assay (ELISA) and the results showed that 46 (54%) of the rose Bengal test samples were positive and 39 (46%) were negative for bcsp31 (p ≤ 0.05) and used as the gold standard for all of the comparisons in this study. The ELISA results indicate that the mean concentration of MIF was significantly higher in patients with positive rose Bengal tests when compared to the control groups and that its concentration increases with increasing age in both the patient and control groups (p ≤ 0.05).

Keywords: Brucellosis, MIF, Iraq, cytokine, bcsp31

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