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Microbiology and Biotechnology Letters

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Molecular and Cellular Microbiology  |  Functional Genomics and Systems Biology

Microbiol. Biotechnol. Lett. 2020; 48(4): 556-563

https://doi.org/10.48022/mbl.2006.06003

Received: June 9, 2020; Accepted: September 7, 2020

단풍잎돼지풀 발효 추출물의 항산화 효과 및 B16F10 세포에서의 미백 활성 검증

Verification of the Antioxidant Effects and Whitening Activity of fermented Ambrosia trifida L. Extracts in B16F10 Cells

Dan-Hee Yoo1,2, Min-Jeong Oh1, Hyeon-Ji Yeom1 and Jin-Young Lee1*

1Division of Cosmetics Biotechnology, Hoseo University, Chungnam 31499, Republic of Korea 2Industry-Academic Collaboration Founation, Seowon University, Cheongju 28674, Republic of Korea

Correspondence to :
Jin-Young  Lee,     jylee@hoseo.edu

The purpose of this study was to verify the antioxidant and whitening effects of fermented Ambrosia trifida L. extract (ATFE) and to verify its usefulness as a cosmetic material. The antioxidant effects were measured by assessing the electron-donating capacity and 2,2-azino-bis(3-ethyl-benthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability of these extracts. ATFE was shown to have an electron-donation capacity of 68.4% at a concentration of 1000 μg/ml. While its ABTS+ radical scavenging ability was shown to be 58.7% at the same concentration. The ATFE tyrosinase inhibitory effect, which is related to skin-whitening, was shown to be 32.35% at a concentration of 1000 μg/ml and a cell viability assay using melanoma cells showed a 14.8% reduction in cell viability at a concentration of 100 μg/ml. Surviving cells were then used in western blot analyses to evaluate the protein inhibitory effects of ATFE at 25, 50, 100 μg/ml where β-actin was used as a positive control. The whitening effects of these extracts were also evaluated by western blot and show that the expression of microphthalmia-associated transcription factors, Tyrosinase-related proteins (TRP)-1, TRP-2 and Tyrosinase were all inhibited, 51.14%, 55.4%, 38.6%, 83.77% respectively, at 100 μg/ml ATFE. The efficacy of the whitening effects was verified and the suitability of ATFE as a cosmetic material was assured.

Keywords: Ambrosia trifida L., fermentation, antioxidant, whitening effect

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