Characterization of Cellular Growth, CO2 Assimilation and Neutral Lipid Production for 4 Different Algal Species

Chae Yoon Shin; Young Jin Noh; So-Yeon Jeong and Tae Gwan Kim

doi:10.48022/mbl.2008.08017

보문(Article)

Environmental Microbiology | Biodegradation and Bioremediation

Microbiol. Biotechnol. Lett. 2020; 48(4): 547-555

https://doi.org/10.48022/mbl.2008.08017

Received: September 2, 2020; Accepted: October 13, 2020

미세조류 4종의 성장, CO₂ 동화 및 지질 생성 특성

Characterization of Cellular Growth, CO₂ Assimilation and Neutral Lipid Production for 4 Different Algal Species

Chae Yoon Shin, Young Jin Noh, So-Yeon Jeong^* and Tae Gwan Kim^*

Department of Microbiology, Pusan National University, Pusan 46241, Republic of Korea

Correspondence to :
So-Yeon Jeong, jeongsy@pusan.ac.kr
Tae Gwan Kim, tkim@pusan.ac.kr

Abstract

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Microalgae are a promising resource in energy and food production as they are cost-effective for biomass production and accumulate valuable biological resources. In this study, CO₂ assimilation, biomass, and lipid production of 4 microalgal species (Chlorella vulgaris, Mychonastes homosphaera, Coelastrella sp., and Coelastrella vacuolata) were characterized at different CO₂ concentrations ranging from 1% to 9%. Microscopic observation indicated that C. vulgaris was the smallest, followed by M. homosphaera, C. vacuolata, and Coelastrella sp. in order of size. C. vulgaris grew and consumed CO₂ more rapidly than any other species. C. vulgaris exhibited a linear increase in CO₂ assimilation (up to 9.62 mmol·day-¹·l^-1) as initial biomass increased, while the others did not (up to about 3 mmol·day-¹·l^-1). C. vulgaris, Coelastrella sp., and C. vacuolata showed a linear increase in the specific CO₂ assimilation rate with CO₂ concentration, whereas M. homosphaera did not. Moreover, C. vulgaris had a greater CO₂ assimilation rate compared to those of the other species (14.6 vs. ≤ 11.9 mmol·day^-1·l^-1). Nile-red lipid analysis showed that lipid production per volume increased linearly with CO₂ concentration in all species. However, C. vulgaris increased lipid production to 18 mg·l^-1, compared to the 12 mg·l^-1 produced by the other species. Thus, C. vulgaris exhibited higher biomass and lipid production rates with greater CO₂ assimilation capacity than any other species.

Keywords: Microalgae, biomass, CO₂ assimilation, lipid production

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Shin CY, Noh YJ, Jeong SY, Kim TG. Characterization of Cellular Growth, CO<sub>2</sub> Assimilation and Neutral Lipid Production for 4 Different Algal Species. Kor. J. Microbiol. Biotechnol 2020;48:547-555. https://doi.org/10.48022/mbl.2008.08017

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Shin CY, Noh YJ, Jeong SY, Kim TG. Characterization of Cellular Growth, CO<sub>2</sub> Assimilation and Neutral Lipid Production for 4 Different Algal Species. Microbiol. Biotechnol. Lett. 2020;48:547-555.
https://doi.org/10.48022/mbl.2008.08017

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보문(Article)

미세조류 4종의 성장, CO₂ 동화 및 지질 생성 특성