Contact us
닫기
Article Search
닫기

Microbiology and Biotechnology Letters

보문(Article)

View PDF

Microbiol. Biotechnol. Lett. 2020; 48(4): 525-532

https://doi.org/10.48022/mbl.2008.08011

Received: August 19, 2020; Accepted: September 4, 2020

Streptococcus zooepidemicus 유래 히알루론산의 경제적 생산을 위한 연속배양 공정 개발

Development of Continuous Culture Process for Economic Production of Hyaluronic Acid (HA) Biosynthesized by Streptococcus zooepidemicus

Sooyeon Kim and Gie-Taek Chun*

College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea

Abstract

Go to

A continuous fermentation process was carried out to enhance hyaluronic acid (HA) production using Streptococcus zooepidemicus cells. During the 1st stage continuous operation from 8 h with a dilution rate of 0.029/h (D1), HA was produced in the range of 7.5−10 g/l. During the 2nd stage from 44 h with a dilution rate of 0.036/h (D2), HA production (8.28 g/l) was initially reduced to a small extent due to increase of dilution rate from D1 to D2, and then a new pseudo-steady state was formed within a few hours with a concurrent small variations of HA production. The HA amount produced during the latter part of the 2nd stage was stably maintained in the range of 8.28−9.48 g/l, about 4.7% less amount compared to the 1st stage. Due to 24% increase of dilution rate from D1 to D2, however, maximum volumetric productivity (DP) amounting to 0.341 g/l/h was obtained at 96 h during the 2nd stage. This maximum productivity obtained from the continuous culture turned out only a small increase (3%) as compared to the corresponding batch fermentation. However, it should be noted that, in the case of batch process, one run typically consists of serial stages of growth culture plus one final production culture. This implies that, if the continuous fermentation that practically needs no dead time necessary for the multi-stage growth cultures is run for longer period, the total amount of the accumulated HA would be far greater than the amount obtained from the corresponding batch culture performed for the identical period.

Keywords: Hyaluronic acid, Streptococcus zooepidemicus, batch fermentation, continuous culture, dilution rate, steady state

References

Go to
  1. Kogan G, Šoltés L, Stern R, Gemeiner P. 2007. Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications. Biotechnol. Lett. 29: 17-25.
    Pubmed CrossRef
  2. Schanté CE, Zuber G, Herlin C, Vandamme TF. 2011. Chemical modifications of hyaluronic acid for the synthesis of derivatives for a broad range of biomedical applications. Carbohydr. Polym. 85: 469-489.
    CrossRef
  3. Kim KH, Kim KT, Kim YH, Kim JG, Han CS, Park SH, Lee BY. 2007. Preparation of oligo hyaluronic acid by hydrolysis and its application as a cosmetic ingredient. J. Soc. Cosmet. Sci. Korea 33:189-196.
  4. Liu L, Liu Y, Li J, Du G, Chen J. 2011. Microbial production of hyaluronic acid: current state, challenges, and perspectives. Microb. Cell Fact. 10: 99.
    Pubmed KoreaMed CrossRef
  5. Schiraldi C, La Gatta A, De Rosa M. 2010. Biotechnological production and application of hyaluronan. Biopolymers. 20: 387-412.
    CrossRef
  6. Kim SJ, Park SY, Kim CW. 2006. A novel approach to the production of hyaluronic acid by Streptococcus zooepidemicus. J. Microbiol. Biotechnol. 16: 1849-1855.
  7. Duan XJ, Niu HX, Tan WS, Zhang X. 2009. Mechanism analysis of effect of oxygen on molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. J. Microbiol. Biotechnol. 19:299-306.
  8. Duan XJ, Yang L, Zhang X, Tan WS. 2008. Effect of oxygen and shear stress on molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. J. Microbiol. Biotechnol. 18:718-724.
  9. Zhang X, Duan XJ, Tan WS. 2010. Mechanism for the effect of agitation on the molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. Food Chem. 119: 1643-1646.
    CrossRef
  10. Pedersen MB, Gaudu P, Lechardeur D, Petit MA, Gruss A. 2012. Aerobic respiration metabolism in lactic acid bacteria and uses in biotechnology. Annu. Rev. Food Sci. Technol. 3: 37-58.
    Pubmed CrossRef
  11. Cleary PP, Larkin ANNICE. 1979. Hyaluronic acid capsule: strategy for oxygen resistance in group A streptococci. J. Bacteriol. 140: 1090-1097.
    Pubmed KoreaMed CrossRef
  12. Condon S. 1987. Responses of lactic acid bacteria to oxygen. FEMS Microbiol. Rev. 3: 269-280.
    CrossRef
  13. Kim SY. 2015. Strain Improvement and Bioprocess Optimization for Industrial Production of Hyaluronic Acid. M.S. Kangwon National University. Republic of Korea.
  14. Herbert D, Elsworth R, Telling RC. 1956. The continuous culture of bacteria; a theoretical and experimental study. Microbiology 14: 601-622.
    Pubmed CrossRef
  15. Blank LM, McLaughlin RL, Nielsen LK. 2005. Stable production of hyaluronic acid in Streptococcus zooepidemicus chemostats operated at high dilution rate. Biotechnol. Bioeng. 90: 685-693.
    Pubmed CrossRef
  16. 張利燮, 鄭敎民. 1987. Chemostat Culture 에서의 Hyaluronic Acid 生産에 關한 硏究. KSBB J. 1: 25-28.(영어로 해주세요)
  17. Ellwood DC, Evans CGT, Dunn GM, McInnes N, Yeo RG, Smith KJ, 1995. Fermentech Medical Ltd., Edinburgh, UK, assignee. Production of hyaluronic acid. US Patent 5,411,874.
  18. Ståhl, S. 2003. U.S. Patent No. 6,537,795. Washington, DC: U.S. Patent and Trademark Office.
  19. Bitter T. 1962. A modified uronic acid carbazole reaction. Anal. Biochem. 4: 330-334.
    CrossRef
  20. Chen SJ, Chen JL, Huang WC, Chen HL. 2009. Fermentation process development for hyaluronic acid production by Streptococcus zooepidemicus ATCC 39920. Korean J. Chem. Eng. 26:428-432.
    CrossRef
  21. Shah MV, Badle SS, Ramachandran KB. 2013. Hyaluronic acid production and molecular weight improvement by redirection of carbon flux towards its biosynthesis pathway. Biochem. Eng. J. 80: 53-60.
    CrossRef

Article Tools

Starts of Metrics

Share this article on :

  • mail

Related articles in MBL

Most KeyWord ?

What is Most Keyword?

  • It is most registrated keyword in articles at this journal during for 2 years.