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Microbiology and Biotechnology Letters

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Microbial Biotechnology  |  Protein Structure, Function, and Engineering

Microbiol. Biotechnol. Lett. 2020; 48(4): 463-470

https://doi.org/10.48022/mbl.2005.05007

Received: May 11, 2020; Accepted: June 9, 2020

Trametes velutina JS18 유래 멜라닌 탈색 효소의 생산, 정제 및 특성

Production, Purification and Characterization of a Melanin Bleaching Enzyme from Trametes velutina JS18

Sung-Jong Jeon1,2* and Tae-Yun Kim1

1Biomedical Engineering and Biotechnology Major, Division of Applied Bioengineering, 2Department of Smart-Biohealth, Dong-Eui University, Busan 47340, Republic of Korea

Correspondence to :
Sung-Jong Jeon,    jeon.sj@deu.ac.kr

The JS18 strain was isolated from an old tree forest and produced extracellular enzymes that decolorize synthetic melanin. Phylogenetic analysis, based on the internal transcribed spacer (ITS) sequence, indicate that JS18 belongs to the Trametes velutina species. JS18 demonstrated laccase activity but no manganese peroxidase or lignin peroxidase activity. Batch culture indicated that the melanin decolorization activity of JS18 strain originated from the laccase. Syringic acid and CuSO4 induced maximum laccase production, yielding 98 U/mL laccase activity after cultivation for 7 days at 25℃. T. velutina secretes an extracellular laccase in GYP medium, and this enzyme was purified using (NH4)2SO4 precipitation, Hi-trap Q Sepharose columns and gel filtration. The molecular weight of the purified enzyme was estimated to be 67 kDa using sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme produced 80% of its melanin decolorization activity within the first 24 h of evaluation in the presence of 1-hydroxybenzotriazole (HBT), while only about 4% of the melanin was decolorized in the absence of the mediator. The greatest decolorization was observed at 1.5 mM/l HBT, which decolorized 81% of the melanin within the first 24 h. The optimum pH and temperature for this decolorization were found to be 5.0 and 37℃, respectively. Our results suggest the possibility of applying HBT induced T. velutina JS18 laccase-catalyzed melanin decolorization.

Keywords: Melanin, decolorization, Trametes velutina, laccase

  1. Lin WP, Lai HL, Liu YL, Chiung YM, Shiau CY, Han JM, et al. 2005. Effect of melanin produced by a recombinant Escherichia coli on antibacterial activity of antibiotics. J. Microbiol. Immunol. Infect. 38: 320-326.
  2. Butler MJ, Day AW. 1998a. Destruction of fungal melanins by ligninases of Phanerochaete chrysosporium and other white rot fungi. Int. J. Plant Sci. 159: 989-995.
    CrossRef
  3. Butler MJ, Day AW. 1998b. Fungal melanins: a review. Can. J. Microbiol. 44: 1115-1136.
    CrossRef
  4. Riley PA. 1997. Melanin. Int. J. Biochem. Cell Biol. 29: 1235-1239.
    CrossRef
  5. Henson JM. 2001. Melanin, pp. 229-246. In M. Hofrichter and A. Steinbuechel (ed.), Biopolymers, Vol.1, Wiley-VCH Verlag GmbH, Weinheim, Germany.
    CrossRef
  6. Terao M, Tomita K, Oki T, Tabe L, Gianni M, Garattini E. 1992. Inhibition of melanogenesis by BMY-28565, a novel compound depressing tyrosinase activity in B16 melanoma cells. Biochem. Pharmacol. 43: 183-189.
    CrossRef
  7. Boissy RE, Visscher M, De Long MA. 2005. DeoxyArbutin: a novel reversible tyrosinase inhibitor with effective in vivo skin lightening potency. Exp. Dermatol. 14: 601-608.
    Pubmed CrossRef
  8. Kim YJ, Uyama H. 2005. Tyrosinase inhibitors from natural and synthetic sources: structure, inhibition mechanism and perspective for the future. Cell Mol. Life Sci. 62: 1707-1723.
    Pubmed CrossRef
  9. Rendon MI, Gaviria JI. 2005. Review of skin‐lightening agents. Dermatologic Surgery 31: 886-890.
    Pubmed CrossRef
  10. Matsubayashi T, Sakaeda T, Kita T, Kurimoto Y, Nakamura T, Nishiguchi K, et al. 2003. Intradermal concentration of hydroquinone after application of hydroquinone ointments is higher than its cytotoxic concentration. Biol. Pharm. Bull. 26:1365-1367.
    Pubmed CrossRef
  11. Del Giudice P, Yves P. 2002. The widespread use of skin lightening creams in Senegal: a persistent public health problem in West Africa. Int. J. Dermatol. 41: 69-72.
    Pubmed CrossRef
  12. Al-Saleh I, Shinwari N, El-Doush I, Billedo G, Al-Amodi M, Khogali F. 2004. Comparison of mercury levels in various tissues of albino and pigmented mice treated with two different brands of mercury skinlightening creams. Biometals 17: 167-175.
    Pubmed CrossRef
  13. Kaneko S, Cheng M, Murai H, Takenaka S. 2009. Purification and characterization of an extracellular laccase from Phlebia radiata strain BP-11-2 that decolorizes fungal melanin. Biosci. Biotechnol. Biochem. 73: 939-942.
    Pubmed CrossRef
  14. Khammuang S, Sarnthima R. 2013. Decolorization of synthetic melanins by crude laccases of Lentinus polychrous Lév. Folia microbiologica 58: 1-7.
    Pubmed CrossRef
  15. Nagasaki K, Kumazawa M, Murakami S, Takenaka S, Koike K, Aoki K. 2008. Purification, characterization, and gene cloning of Ceriporiopsis sp. strain MD-1 peroxidases that decolorize human hair melanin. Appl. Environ. Microbiol. 74: 5106-5112.
    Pubmed KoreaMed CrossRef
  16. Kim BS, Blaghen M, Hong HS, Lee KM. 2013. Purification and characterization of a melanin biodegradation enzyme from Geotrichum sp. Int. J. Cos. Sci. 38: 622-626.
    Pubmed CrossRef
  17. Woo SH, Cho JS, Lee BS, Kim EK. 2004. Decolorization of melanin by lignin peroxidase from Phanerochaete chrysosporium. Biotechnol. Bioprocess. Eng. 9: 256-260.
    CrossRef
  18. Mohorčič M, Friedrich J, Renimel I, Andre P, Mandin D, Chaumont JP. 2007. Production of melanin bleaching enzyme of fungal origin and its application in cosmetics. Biotechnol. Bioproce. Eng. 12: 200-206.
    CrossRef
  19. Hervé M, Hirschinger J, Granger P, Gilard P. 1994. A 13C solidstate NMR study of the structure and auto-oxidation process of natural and synthetic melanins. Biochi. Biophy. Acta. 1204: 19-27.
    CrossRef
  20. Coconi-Linares N, Magaña-Ortíz D, Guzmán-Ortiz DA, Fernández F, Loske AM, Gómez-Lim MA. 2014. High-yield production of manganese peroxidase, lignin peroxidase, and versatile peroxidase in Phanerochaete chrysosporium. Appl. Microbiol. Biotechnol. 98: 9283-9294.
    Pubmed CrossRef
  21. Archibald FS. 1992. Lignin peroxidase activity is not important in biological bleaching and delignification of unbleached kraft pulp by Trametes versicolor. Appl. Environ. Microbiol. 58: 31013109.
    Pubmed KoreaMed CrossRef
  22. Baldrian P. 2006. Fungal laccases - occurrence and properties. FEMS Microbiol. Rev. 30: 215-242.
    Pubmed CrossRef
  23. Cho JS. 2002. Studied on decoloration of melanin using laccase. Available from http://dspace.inha.ac.kr/handle/10505/11037.
  24. Son MJ, Kim YH, Nam SW, Jeon SJ. 2019. Optimization of Media Composition on the Production of Melanin Bleaching Enzyme from Peniophora sp. JS17. Microbiol. Biotechnol. Lett. 47: 250258.
    CrossRef
  25. Yang Y, Wei F, Zhuo R, Fan F, Liu H, Zhang C, et al. 2013. Enhancing the laccase production and laccase gene expression in the white-rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds. PLoS One 8: e79307.
    Pubmed KoreaMed CrossRef
  26. Tavares APM, Coelho MAZ, Agapito MSM, Coutinho JAP, Xavier AMRB. 2006. Optimization and modeling of laccase production by Trametes versicolor in a bioreactor using statistical experimental design. Appl. Biochem. Biotech. 134: 233-248.
    CrossRef
  27. Rosales E, Couto SR, Sanromán MA. 2007. Increased laccase production by Trametes hirsuta grown on ground orange peelings. Enzy. Microb. Technol. 40: 1286-1290.
    CrossRef
  28. Xu F, Kulys JJ, Duke K, Li K, Krikstopaitis K, Deussen HJ, et al. 2000. Redox chemistry in laccase-catalyzed oxidation of Nhydroxy compounds. Appl. Environ. Microbiol. 66: 2052-2056.
    Pubmed KoreaMed CrossRef

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